Session Index

Optical sectioning techniques offer three-dimensional information from tissues, but require individual axial planes to be imaged consecutively. We introduce active illumination, utilizing speckle or Talbot effect, and multiplexed volume holography, demonstrating three-dimensional biopsy for microscopy as well as endoscopy, without scanning.

 

In digital holography, the recording of off-axis object light is a challenge because of the limited sampling rate. Here we proposed to apply optical scanning holography (OSH) to realize the recording of off-axis object light. The reconstruction method is also discussed.

 

A novel phase conjugation mechanism via linear medium is proposed. This technique is applied to digital holographic microscopy. On pure optical basis, the technique showed a low spatial standard deviation, which proves its effectiveness in noise suppression.

 

We present a differential phase contrast volume holographic microscopy (DPC-VHM) based on asymmetric illumination to simultaneously obtain multi-depth phase contrast images of a biological samples in one shot, using LED illumination. Experimental results demonstrate the imaging of the proposed system to observe the bright field and DPC imaging.

 

A three-dimensional sample rotation method is proposed, by combining digital holographic microscopy and holographic optical tweezer. The sample’s shape, size, and the phase-centroid are estimated using DHM and this information is used in Gerchberg-Saxton algorithm to generate computer-generated holograms to manipulate the trapping beam in three-dimensions for stable sample rotation.